A DNA profile taken from underneath Ashling Murphy's fingernails matched that of Jozef Puska, the man accused of her murder, a scientist has told the Central Criminal Court.
Dr Lorna Flanagan on Friday told prosecution counsel Anne-Marie Lawlor SC she received swabs that had been taken from under the nails of each of Ms Murphy's hands during a post-mortem carried out by State Pathologist Dr SallyAnne Collis.
Dr Flanagan isolated the male DNA present in the swabs by focusing only on the y-chromosome to eliminate Ms Murphy's DNA from her results.
She compared the DNA to a blood sample belonging to Mr Puska and from swabs that were taken from Mr Puska when he was in custody in Tullamore Garda Station on January 18 last year.
She found that the Y-STR profile generated from the nail swabs matched the Y-STR profile from Mr Puska's blood sample and swabs.
Dr Flanagan said that she estimated the chances of finding the same male-specific DNA profile from someone unrelated to Mr Puska is approximately one in 14,000 [one in fourteen thousand].
She explained that those statistics are calculated from a database of western European ethnic groups that includes people from Ireland and as far east as Latvia, Ukraine and Romania. Dr Flanagan told defence counsel Michael Bowman SC that the groups include people from Slovakia and members of the Roma community.
Jozef Puska (33), with an address at Lynally Grove, Mucklagh, Co Offaly, has pleaded not guilty to murdering Ms Murphy at Cappincur, Tullamore, Co Offaly on January 12th, 2022.
Det Insp Brian Farrell told defence counsel Michael Bowman SC that he was the joint Senior Investigating Officer coordinating the garda investigation into Ms Murphy's death. He agreed with Mr Bowman that following Ms Murphy's death on January 12th last year they were "dealing with a horrendous fatality, a murder" and they were "acting as quickly as possible" to find the person responsible.
He agreed that a man was arrested shortly after 4pm that day but that person was eliminated as an active suspect some time after 9pm by DNA evidence. A further "significant development" came when the decision was taken to send Det Sgt Brian Jennings and Det Gda Fergus Hogan from Tullamore to St James's Hospital in Dublin to speak to Mr Puska.
The inspector agreed that in his notes made during that period he repeatedly referred to Mr Puska as a "suspected offender". He said he does not believe he told the members who had been sent to Dublin that the first suspect had been released. "It wouldn't be something I would usually do because it's not relevant to their task," he said.
At that time, he said the investigation was "fast moving, very dynamic" and they were taking in a lot of information.
Part of the task for the two gardai who were sent to meet Mr Puska, he said, was to talk to him and to "have a look at him" but he couldn't remember if he "specifically asked" gardai to look at Mr Puska or to compare him to a person captured on CCTV images that gardai had gathered in their investigation.
The inspector said that the notes he has from the time show that he wanted gardai to get an account from Mr Puska of his movements on the 12th and to ask if he owns a bicycle.
He also had a note that Mr Puska had three stab wounds, one of which was close to his liver and that he would be in hospital for about one week. He said it "would make sense" that Det Gda Hogan gave him the information about Mr Puska's condition.
He further agreed that he directed the detectives to remain in the area of St James's Hospital and over the following days he made inquiries about when Mr Puska was to be released from hospital.
Superintendent Pat O'Callaghan told Ms Lawlor that he was the Senior Investigating Officer in relation to Ms Murphy's death. He said there were more than 720 jobs created by the incident room for members to carry out.
He agreed that a man was arrested on January 12th and released on January 13th at about 10.30pm. Superintendent O'Callaghan said the man in question gave a consistent account of his movements on the day which were corroborated by his mother and grandmother.
On the late afternoon of January 13th, he said, it became apparent that this man was not involved. His fingerprints did not match those taken from a bicycle found near the scene where Ms Murphy was killed and his DNA did not match DNA that was taken from the scene.
He said that the manner in which Ms Murphy met her death had not been put into the public domain when Mr Puska told Det Gda Fergus Hogan in St James's Hospital in Dublin on January 14th that he had "cut Ms Murphy". Superintendent O'Callaghan said he had not told Det Gda Hogan about how she died because the investigation team had taken a decision not to release that information.
CCTV
The superintendent also confirmed that a significant number of searches were carried out in locations associated with Mr Puska but the clothing he was seen wearing on CCTV footage from the day of Ms Murphy's death were not found.
Supt O'Callaghan told Mr Bowman that he was not aware if information about how Ms Murphy died was circulating on social media. He said there was "nothing released by An Garda Síochána on the cause of death".
He accepted that detectives interviewing the first person identified as a suspect spoke to him about knives. Mr Bowman suggested that information about how Ms Murphy died was "beginning to get out there".
Supt O'Callaghan said the suspect was "interviewed inside in a garda station... by a specific team of detectives and they would have had a specific line of inquiry." He said the cause of death was not known at all until 5pm on January 13th when the pathologist confirmed that Ms Murphy had died from stab wounds.
The superintendent agreed that he would be surprised if the two members sent to Dublin to speak to Mr Puska did not understand that they were there to "look at the suspect".
Det Sgt David Scahill told Ms Lawlor that he was sent to St James's on January 18 last year to arrest Mr Puska. He carried out the arrest at 10.31am and explained the reason for the arrest. Mr Puska indicated that he understood and was taken to Tullamore Garda Station, arriving at 12.30pm.
The following day Det Insp Brian Farrell told Det Sgt Scahill that the Director of Public Prosecutions had directed that Mr Puska be charged with murder. At 7.22pm that evening Det Sgt Scahill charged Mr Puska with the murder of Ashling Murphy and cautioned him. Mr Puska replied: "No."
Det Gda Scahill told Mr Bowman that the intended arrest of Mr Puska was not widely known. He said that he does not recall there being a big crowd of people at the front of Tullamore Garda Station when they arrived but added that he went in through the back, not the front. He said that when Mr Puska was searched in Tullamore he had no property on him.
Gda Ciara Martin was the member in charge of Tullamore Garda Station when Mr Puska was detained. She also told Mr Bowman that she did not notice a crowd of people at the front of the station. "If it happened, I wasn't aware," she said.
The trial continues before Mr Justice Tony Hunt and a jury of nine men and three women.
