The latest figures from the Irish Nurses and Midwives Organisation (INMO) show there is 515 patients waiting for beds in Irish hospitals.
University Hospital Limerick once again has the highest number of people on trolleys, with 102 patients, with 47 of those in the Emergency Department of the hospital.
Cork University Hospital has 72 patients on trolleys, with 60 people in the Emergency Department.
University Hospital Galway has 40 patients on trolleys, with 31 of those patients in the Emergency Department.
Wednesday's figures come as the INMO figures for April showed there was 11,070 patients on trolleys in April, with University Hospital Limerick having 1,971 patients without a bed through the month.
The INMO Trolley Watch counts the number of patients who have been admitted to acute hospitals, but who are waiting for a free bed.
These patients are often being treated on trolleys in corridors, "but they may also be on chairs, in waiting rooms, or simply wherever there’s space". The INMO started Trolley Watch in 2004.