Wearing a mask and handcuffed, Samantha Jones, 51, was escorted by police into the courthouse in Alor Setar in the northern state of Kedah.
She was charged after police found a blood-stained kitchen knife in the couple’s home where John William Jones was found dead on October 18, 2018.
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Police have said Jones confessed she stabbed her husband in the chest during a heated argument.
The couple moved to tropical Langkawi island 11 years ago under the Malaysia My Second Home programme, which gives foreigners long-staying visas.
A conviction for murder carries a mandatory death sentence by hanging.